PDE2A (Rat), His-GST-tags Recombinant

Catalog #

60022

$455 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Rat PDE2A (GenBank Accession No. NM_00143847), full length, with N-terminal His-GST-tag, MW=133 kDa, expressed in a Baculovirus infected Sf9 cell expression system

This product has been cited 4 times.

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Synonyms

PDE2A, phosphodiesterase 2A, PDE2-A, PDE

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations4

Species

Rat

Host Species/Expression System

Sf9 insect cells

Purity

≥75%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol, and 3 mM DTT

133 kDa

Amino Acids

full length

Specific Activity

≥ 2500 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Genbank #

NM_001143847

UniProt #

Q01062

Tag(s)

N-terminal His-GST-tags

References

1. Seybold, J., et al., Blood 105 (9), 3569- 3576 (2005).
2. Martinez, S.E., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (20), 13260-13265 (2002).

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE2A at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).

Scientific Category

PDE

Regulatory Status

Research Use Only