Rat PDE2A, His-GST-tags

Catalog #: 60022
Size: 5 µg
$425
Species: Rat
Host Species: Sf9 insect cells
MW: 133 kDa
Genbank #: NM_001143847
Tag(s): N-terminal His-GST-tags
a.a: full length
Description:
Rat PDE2A (GenBank Accession No. NM_00143847), full length, with N-terminal His-GST-tag, MW=133 kDa, expressed in a Baculovirus infected Sf9 cell expression system
UniProt
Q01062
Synonym(s):
PDE2A, phosphodiesterase 2A
Specific Activity:
≥ 2500 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay Conditions:
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE2A at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol, and 3 mM DTT
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Seybold, J., et al., Blood 105 (9), 3569- 3576 (2005).
2. Martinez, S.E., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (20), 13260-13265 (2002).

Application Reference(s):
A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013)

Warning(s):
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Scientific Category:
PDE
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

60022_140523-G1.pdf