PDE1C TR-FRET Assay Kit

Catalog #
60705
$405 *
Size: 96 reactions
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*US Pricing only. For international pricing, please contact your local distributor.
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Description

Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE1C is a calmodulin-dependent PDE that is expressed principally in human myocardium. The PDE1C TR-FRET Assay Kit is designed for identification of inhibitors of PDE1C using TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) technology. The assay is based on the generation of FAM-labeled nucleotide monophosphates by the PDE1C. These phosphate groups bind to terbium-labeled nanoparticles, resulting in energy transfer from the terbium to the FAM, which emits a fluorescent signal at 520 nm. The change in fluorescent intensity can be easily measured using a fluorescence plate reader.

Using this kit, only two simple steps on a microtiter plate are required for the PDE1C activity assay. First, the fluorescent-labeled cAMP is incubated with a sample containing PDE1C for 1 hour. Second, a binding agent and a terbium donor are added to the reaction mix and incubated for 30 minutes. Then, fluorescence intensity can be measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against PDE1C? Check out our Phosphodiesterase Screening Services.

Synonyms
inhibitor screening, FRET assay kit, PDE1C, HTRF, PDE1-C, PDE
Product Info
Storage and Usage
Citations
Assay Kit Format
TR-FRET
Supplied As
The PDE1C TR-FRET Assay Kit comes in a convenient 96-well format, with purified PDE1C enzyme, fluorescently labeled PDE1C substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions.
Format
Catalog # Component Amount  Storage
60013 PDE1C recombinant enzyme 1 µg -80°C

Avoid freeze/
thaw

cycles!

60200 FAM-Cyclic-3′, 5′-AMP: 20 µM 20 µl -80 °C
60393 PDE assay buffer 25 ml -20 °C
60394 Tb donor 50 µl -80 °C
60390 Binding Agent 200 µl +4 °C
78422 Binding Buffer A 20 ml +4 °C
78423 Binding Buffer B 20 ml +4 °C
79685 Black, low binding NUNC microtiter plate 1 Room. Temp

 

UniProt #
Q14123
References
Vandeput. F., et al. J. Biol. Chem. 2007; 282(45): 32749-32757.