PDE1C, GST-Tag Recombinant

Catalog #

60013

$455 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human recombinant full length PDE1C (phosphodiesterase 1C), containing amino acids 2-634(end). This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified.

This product has been cited 2 times.

●

Synonyms

Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C, Cam-PDE 1C, Hcam3, PDE1-C, PDE

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PDE1C Activity

PDE1C inhibition curves using a fluorescence polarization assay (#60312). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations2

Species

Human

Construct

PDE1C (GST-2-634(end))

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.04% Tween-20, and 20% glycerol

99 kDa

Amino Acids

2–634(end)

Specific Activity

≥15,000 pmol/min/µg

Genbank #

NM_005020

UniProt #

Q14123

Tag(s)

N-terminal GST-tag

Background

Phosphodiesterases (PDEs) play an important role in the dynamic regulation of the second messengers cAMP (cyclic adenosine monophosphate) and cGMP (cyclic guanosine monophosphate) signaling, by hydrolyzing them. The PDE superfamily is composed of 11 families, with PDE4, 7 and 8 being cAMP-specific hydrolases, and thus regulating positive and negative responses to it. PDE1 is encoded by 3 genes, PDE1A-C, and has multiple splice variants. Human PDE1C can be found in several tissues, including the brain, heart, smooth muscle cells (SMCs), cardiomyocytes and pancreas. PDE1C is involved in multiple biological functions, in a cell-specific context. It is involved in SMC proliferation and collagen deposition, being highly expressed in the myocardium. It can form a complex with A₂ Receptor and TRPC3 (transient receptor potential channel 3), serving as an antagonist for A₂ Receptor related pathways, and leading to apoptosis. Inhibition of PDE1C can thus be a strategy to fight vascular aging and doxorubicin-induced cardiotoxicity. It also participates in stress related release of CRF (corticotrophin-releasing factor) and arginine vasopressin and aged related cAMP reduction in the hippocampus. Its various functions make it a highly relevant target for the treatment of many disorders, and the use of PDE1C inhibitors will continue to open new therapeutic avenues.

References

1. Vandeput, F., et al. (2007). J. Biol. Chem. Nov 9;282(45):32749-57.
2. Cygnar, K.D., Zhao, H. (2009). Nat. Neurosci. Apr;12(4):454-62.
3. Ortiz-Capisano, M.C., et al. (2009). Am. J. Physiol. Regul. Integr. Comp. Physiol. Nov;297(5):R1469-76.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed with increasing concentrations of PDE1C in 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.05% Tween-20, 200 µM cGMP, 2.5 kU 5'-nucleotidase, 0.1 mg/ml BSA. The reaction was incubated at 37°C for 20 minutes followed by addition of malachite green reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only