PDE1A, GST-tag Recombinant

Catalog #

60010

$455 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human PDE1A (GenBank Accession No. NM_005019), full length with N-terminal GST-tag, MW= 88.3 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 16 times.

●

Synonyms

PDE1A, phosphodiesterase 1A, PDE1-A, PDE

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE1A inhibition curves using a fluorescence polarization assay (#60310). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations16

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥84%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, and 20% glycerol

88.3 kDa

Amino Acids

full length

Specific Activity

≥ 43,000 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Genbank #

NM_001258313.1

UniProt #

P54750-4

Tag(s)

N-terminal GST-tag

References

1. Nagel, D.J., et al., Circ. Res. 98 (6), 777-784 (2006).
2. Goraya, T.A.., et al., J. Biol. Chem. 279 (39), 40494-40504 (2004).

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE1A at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only