PDE11A, GST-Tag (Mouse) Recombinant

Catalog #

60064

$460 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Mouse PDE11A (GenBank Accession No. NM_001081033), a.a. 2-933(end) with N-terminal GST tag, MW= 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 7 times.

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Synonyms

PDE11, phosphodiesterase 11A, PDE11-A, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations7

Species

Mouse

Host Species/Expression System

Sf9 insect cells

Purity

≥85%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 20 mM Glutathione, 10% glycerol, and 3 mM DTT.

131 kDa

Amino Acids

2-933(end)

Specific Activity

≥ 1,560 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C.

Genbank #

NM_001081033

Tag(s)

N-terminal GST-tag

References

1. Fawcett, L. et al., PNAS. 97(7), 3207-3707 (2000).

2. Horvath, A. et al., Nature Genetics. 38, 794-800 (2006).

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE11A at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only