PDE11A4, GST-tag Recombinant

Catalog #

60110

$455 *

Size: 10 µg

Qty

Bulk/Custom

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This product has been cited 27 times.

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Synonyms

PDE11, phosphodiesterase 11A, PDE11-A4, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE11A4 inhibition curve using a fluorescence polarization assay (#60411). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations27

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 1.6 mM glutathione

131 kDa

Amino Acids

1-943(end)

Specific Activity

1560 pmol/min/µg

Genbank #

BC112393

UniProt #

Q9HCR9

Tag(s)

N-terminal GST-tag

References

1. Loughney K, et al., Int. J. Impot. Res. 2005, 17(4):320-5.
2. Weeks JL, et al., Int. J. Impot. Res. 2005, 17(1):5-9.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE11A4 at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only