PDE10A2, GST-Tag Recombinant

Catalog #

60100

$455 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human PDE10A2 (phosphodiesterase 10A), full length, encompassing amino acids 1-789(end). This construct contains an N-terminal GST-tag followed by a His-tag (6xHis) and a Thrombin Cleavage Site. The recombinant protein was affinity purified.

This product has been cited 6 times.

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Synonyms

PDE10A2, phosphodiesterase 10A2, PDE10-A2, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PDE10A2 Activity

Product Info

Storage and Usage

Citations6

Species

Human

Construct

PDE10A2 (GST-1-789(end))

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween, 3 mM DTT, and 20% Glycerol.

118 kDa

Amino Acids

1-789(end)

Specific Activity

≥1600 pmol/min/µg

Genbank #

NM_006661

UniProt #

Q9Y233

Tag(s)

N-terminal GST-tag

References

1. Gross-Langenhoff,M., et al., J. Biol. Chem. 281 (5), 2841-2846 (2006).
2. Frame,M., et al., Cell. Signal. 13 (10), 735- 741 (2001).

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE10A2 at 37°C for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only