PDE10A1, GST-tag Recombinant

Catalog #

60099

$455 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human PDE10A1 (GenBank Accession No. NM_001130690), a.a. 2-789(end) with N-terminal GST-tag, MW=116 kDa, expressed in Sf9 cells via a Baculovirus expression system.

This product has been cited 15 times.

●

Synonyms

PDE10A1, phosphodiesterase 10A1, PDE10-A1, PDE

●

Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations15

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥48%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 20 mM glutathione, 3 mM DTT and 10% glycerol.

116 kDa

Amino Acids

2–789(end)

Specific Activity

≥ 3200 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3' , 5' -cAMP to 5' -AMP per minute at 37°C.

Genbank #

NM_001130690

UniProt #

Q9Y233 -2

Tag(s)

N-terminal GST-tag

References

1. Gross-Langenhoff,M., et al., J. Biol. Chem. 281 (5), 2841-2846 (2006).
2. Frame,M., et al., Cell. Signal. 13 (10), 735- 741 (2001).

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and 0.25-4 ng/µl PDE10A1 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only