PDE10A (Mouse), GST-tag Recombinant

Catalog #

60101

$455 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Mouse PDE10A GenBank Accession No. NM_011866), full length with N-terminal GST-tag, MW=117 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PDE10, phosphodiesterase 10A, PDE10-A, PDE

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

PDE10A (Mouse) inhibition curve using a fluorescence polarization assay (#79643). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

117 kDa

Amino Acids

full length

Specific Activity

≥ 1,029 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3' , 5' -cAMP to 5' -AMP per minute at 37°C.

Genbank #

NM_011866

UniProt #

Q8CA95

Tag(s)

N-terminal GST-tag

References

1. Siuciak, J.A. et al., Neuropharmacology 51 (2), 374-385 (2006).
2. Hu,H. et al., Eur. J. Neurosci. 20 (12), 3351-3363 (2004).

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5 -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only