PD-1:PD-L2 Cell-Based Inhibitor Screening Assay Kit

Catalog #
79378
$615 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

The PD-1/PD-L2 Inhibitor Screening Cell-Based Assay is a bioluminescent cell-based assay that can be used to screen and profile inhibitors of the PD-1:PD-L2 interaction. The assay consists of two main components:

• Growth-Arrested PD-1 Effector cells (PD-1/NFAT reporter-Jurkat cells): Reporter Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements and also constitutively expressing Human PD-1. These cryopreserved cells are provided in a thaw-anduse format that does not require cell propagation. These cells are modified not to be expanded and are intended to be used in a single experiment.

• Expression vectors for TCR activator and Human PD-L2: Transfection-ready vectors are used to transfect cells to create the target cells that overexpress PD-L2 and an engineered cell surface T cell receptor (TCR) activator.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC, activity assay kit, cell based kit, cell-based assay
Product Info
Storage and Usage
Citations
Assay Kit Format
Luminescent
Supplied As
Each kit contains sufficient reagents for 100 assays using a 96-well plate.
Materials Required But Not Supplied

• HEK293 cell and its growth medium (Thaw medium 1, #60187) or other cell lines

• Transfection reagent for mammalian cell line [We use Lipofectamine™ 2000 (Life technologies #11668027). However, other transfection reagents work equally well.]

• Opti-MEM I Reduced Serum Medium (Life technologies #31985-062)

• 96-well tissue culture-treated white clear-bottom assay plate

• Luminometer

Format

COMPONENTS:

UniProt #
Q15116
Background
Programmed Cell Death Protein 1 (PD-1) is a receptor which is expressed on activated T-cells. Binding of PD-1 to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. The PD-1 ligands are found on most cancers, and PD-1: PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance.