Anti-PD-L1 (CD274) Neutralizing Antibody

Catalog #: 71213
Size: 100 µg
$475
Species: Human
Host Species: Freestyle 293 cells
MW: 50 and 25 kDa for the heavy and light chains, respectively (150 kDa).
Tag(s): None
Clonality: Monoclonal
Isotype: IgG1κ
Description:

PD-L1 Neutralizing Antibody, recombinant humanized monoclonal, recognizing the extracellular domain of human PD-L1 and blocking the binding of PD-L1 to PD-1. This antibody has been shown to cross-react with mouse PD-L1. No other species have been tested.

Synonym(s):
PD-L1 Neutralizing Antibody, Programmed Cell Death 1 Ligand 1, PDL1, PD-L1, B7 homolog 1, B7H1, B7-H1, CD274, PDCD1L1
Endotoxin Level:
<1 EU/µg
Specific Activity:
IC50 = 11.5 nM (human PD-L1) 1.8 nM (mouse PD-L1)
Assay Conditions:
11.5 nM using PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit (BPS Bioscience, Cat. #72003) and human PD-1 and PD-L1. 1.8 nM using mouse PD-L1 and mouse PD- 1[Biotinylated].
Formulation:
8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Purification:
Protein A affinity chromatography from HEK293 supernatants.
Format:
Aqueous buffer solution.
Storage / Stability:
Stable for at least 12 months at -80°C.
Application(s):
Useful for inhibition of PD1:PD-L1 interactions.
Reference(s):
1. Freeman, G.J., et al., J. Exp. Med. 2000; 192(7): 1027-1034.
2. Dong, H., et al., Nat. Med. 1999; 5(12): 1365-1369.

Warning(s):
Avoid freeze/thaw cycles. Store diluted antibody with carrier protein (BSA 0.1 – 0.5%) at 4°C, stable for 2 weeks.
Scientific Category:
Immunotherapy
Product Type:
Antibody
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Product Datasheets

71213 Lot 160711.pdf