PD-L1 (CD274), Fc fusion (Human) HiP™

Catalog #: 71104-1
Size: 50 µg
$210
Species: Human
Host Species: HEK293
MW: 52 kDa
Genbank #: NM_014143
Tag(s): C-terminal Fc fusion (Human IgG1)
a.a: 19–239
Background:
PD-L1 is involved in the costimulatory signal essential for T-cell proliferation and production of IL-10 and IFNgamma. Interaction with PD-1 inhibits T-cell proliferation and cytokine production, and down-regulates the immune response. PD
Description:

Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7- H1), GenBank Accession No. NM_014143, amino acids 19-239, fused at the C-terminus to the Fc portion of human IgG1, expressed in a HEK293 cell expression system. MW = 52 kDa.

UniProt
Q9NZQ7
Synonym(s):
Programmed Cell Death 1 Ligand 1, PDL1, PD-L1, B7 homolog 1, B7H1, B7-H1, CD274, PDCD1L1
Assay Conditions:
PD-L1 binding to PD-1 was tested following protocol for BPS Cat. #72005. Briefly, wells were coated with 10 ng of PD-L1 in PD-1 assay buffer. Varying concentrations of biotinylated PD-1 was added and wells were incubated at room temp. for 2 hours before adding strep-HRP, followed by chemiluminescent substrate. Luminescence was read on a chemiluminescent plate reader.
Formulation:
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at –80°C.
Application(s):
Useful for the study of protein-protein interaction and in vitro and in vivo protein function.
Reference(s):

1. Freeman, G.J., et al., J Exp Med. 2000 Oct 2;192(7):1027-34.
2. Dong, H., et al., Nat Med. 1999 Dec; 5(12):1365-9.

Application Reference(s):
Cutting Edge: PD-1 Regulates Imiquimod-Induced Psoriasiform Dermatitis through Inhibition of IL-17A Expression by Innate γδ-Low T Cells (2015)

Notes:

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Warning(s):
Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 μg/ml in PBS + glycerol and stored at -80°C.
Scientific Category:
Immunotherapy
Product Type:
Recombinant Protein
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Product Datasheets

71104_Lot150127-D_BCA.pdf