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Description
Recombinant human secreted PD-1 (programmed cell death 1), encompassing amino acids 25-167. The C-terminus is fused with the Fc fragment of a human IgG1 followed by an Avi-Tag™. The recombinant protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified. HiP™ indicates a high purity protein (≥90% pure) and less than 10% aggregation as measured by gel filtration.
This product has been cited 15 times.
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Synonyms
PD1, PD-1, PDCD1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, programmed cell death 1
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Product Data Gallery
PD-1 [Biotinylated]:PD-L1 Activity
Gel Filtration Trace
Biotin-Avidin Pulldown
4-20% SDS-PAGE Coomassie Staining
PD-1[Biotinylated]: PD-L2 inhibition curve using a colorimetric assay (#72019). Representative data only; data may vary by lot.
Product Info
Storage and Usage
Citations15
Construct
PD-1 (25-167-Fc(IgG1)-Avi)-(Biotin)
Host Species/Expression System
HEK293
Glycosylation
This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Label
This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.
For more information on enzymatic biotinylation, please see our Tech Note.
References
1. Shinohara, T., et al., Genomics. 1994 Oct;23(3):704-6.
2. James, E.S., et al., Genes Immun. 2005 Aug;6(5):430-7.
Storage/Stability
At least 6 months at –80°C.
Assay Conditions
Assay was performed according to PD-1 [Biotinylated]:PD-L1 Inhibitor Screening Assay Kit (BPS Bioscience #72005) with various amounts of PD-1 [Biotinylated].
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Shipping Temperature
-80°C
Warnings
Avoid freeze/thaw cycles.
Scientific Category
Immunotherapy
Regulatory Status
Research Use Only