PD-1:PD-L2 TR-FRET Assay Kit

Catalog #: 72012
Size: 384 reactions
$1,040
Background:
Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses.
Description:
The PD-1:PD-L2 TR-FRET Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L2 in a homogeneous 384 reaction format. Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing europium-labeled (Eu) PD-1, dye-labeled acceptor, biotin-labeled PD-L2, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
Supplied As:
This kit comes in a convenient 384-well format, with Europium-labeled PD-1 and biotin-labeled PD-L2.
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt.
Format:

Instructions for use:
See product datasheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
1. Molnar, E., et al. Proc Natl Acad Sci USA. 2008; 105: 10483-10488.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008; 26: 677-704.
Notes:
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Immunotherapy
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

72012.pdf