PD-1:PD-L2[Biotinylated] Inhibitor Screening Assay Kit

Catalog #
72004
$1,095 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

The PD-1:PD-L2[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. The key to this kit is the high sensitivity of detection of biotin-labeled PD-L2 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated on a 96-well plate. Next, PD-L2 is incubated with PD-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Synonyms
activity assay kit, Strep HRP, streptavidin
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Supplied As
Kit comes in a convenient 96-well format, with biotin-labeled PD-L2, purified PD-1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Format
Catalog
Number

Component

Amount

Storage
71108 PD-L2, Biotin-labeled 5 µg -80°C



Avoid
freeze/
thaw
cycles!
71106 PD-1 10 µg -80°C
79742 Streptavidin-HRP 15 ml +4°C
79311 3x Immuno Buffer 1 50 ml -20°C
79728 Blocking Buffer 2 50 ml +4°C

79670
ELISA ECL substrate A
(transparent bottle)
6 ml Room
Temp
ELISA ECL substrate B
(brown bottle)
6 ml Room
Temp
79699 White 96-well microplate 1 +4°C

UniProt #
PD-1: Q15116; PD-L2: Q9BQ51
Background
Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses.
References

1. Molnar, E., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 10483-10488.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.