PD-1:PD-L1 TR-FRET Assay

Catalog #
72038
$845 *
Size: 384 reactions
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Description

The PD-1:PD-L1 TR-FRET Assay is designed to measure the inhibition of PD-1 binding to PD-L1 in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing europium-labeled (Eu) PD-1, dye-labeled acceptor, biotin-labeled PD-L1, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.

Synonyms
Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L, Programmed Cell Death 1 Ligand 1, PDL1, PD-L1, B7 homolog 1, B7H1, B7-H1, CD274, PDCD1L1, Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC, HTRF
Product Info
Storage and Usage
Citations
Assay Kit Format
TR-FRET
Materials Required But Not Supplied

Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

Format
Catalog # Component Amount Storage
  PD-1-Eu  8 µg -80°C

Avoid
freeze/
thaw
cycles!

71105 PD-L1, Biotinylated 30 µg -80°C
  Dye-labeled acceptor 2 x 10 µl -20°C
79311 3x Immuno Buffer 1 4 ml -20°C
  White, non-binding, low volume, 384-well microtiter plate 1 Room
Temp.
UniProt #
PD-1: Q15116; PD-L1: Q9NZQ7
References

1. Molnar, E., et al. Proc Natl Acad Sci USA. 2008; 105: 10483-10488.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008; 26: 677-704.