PD-1:PD-L1[Biotinylated] Inhibitor Screening Colorimetric Assay Kit

Catalog #
72016
$1,210 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD-1:PDL1[Biotinylated] Inhibitor Screening Colorimetric Assay Kit is designed for screening inhibitors of PD-L1 ligand binding to PD-1 receptor. The key to this kit is the high affinity of biotin-labeled PD-L1 for streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated on a 96-well plate. Next, PD-L1 is incubated with PD-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.

This product has been cited 2 times.

Synonyms
Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L and Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7-H1), activity assay kit
Product Info
Storage and Usage
Citations2
Assay Kit Format
Colorimetric
Supplied As
This kit comes in a convenient 96-well format, with biotin-labeled PD-L1, purified PD-1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Materials Required But Not Supplied

PBS (Phosphate buffered saline)
1N HCl (aqueous)
Rotating or rocker platform
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm*
*Alternatively, a spectrophotometer reading at 650 nm may be used, but sensitivity of the assay will be
greatly reduced.

Format
Catalog # Component Amount Storage
71105 PD-L1, Biotin-labeled (Human) 5 µg -80°C Avoid freeze/
thaw cylces
71106 PD-1 (Human) 10 µg -80°C
79742 Streptavidin-HRP 10 µl +4°C
79311 3x Immuno Buffer 1 50 ml -20°C
79728 Blocking Buffer 2 50 ml +4°C
79651 Colorimetric HRP substrate 10 ml +4°C
79964 Transparent 96-well microplate 1 +4°C
UniProt #
PD-1: Q15116; PD-L1: Q9NZQ7
References

1. Lin, D., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 3011-3016.
2. Keir, M.E. et al. Annu. Rev. Immunol. 2008, 26: 677-704.

Application Reference(s):
1.  Selective Suppression of Cell Growth and Programmed Cell Death-Ligand 1 Expression in HT1080 Fibrosarcoma Cells by Low Molecular Weight Fucoidan Extract (2019)