PD-1 / NFAT Reporter - Jurkat Recombinant Cell Line

Catalog #: 60535
Size: 2 vials
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Background:
This PD-1 Cell Line is used to measure the binding of PD-1 to PD-L1/PD-L2. Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T cells, binds to its ligands, PD-L1 and PD-L2, to negatively regulate immune responses. The PD-1 ligands are found on most cancers, and PD-1:PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance. The PD-1:PD-L1/2 pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of cancers, as well as multiple sclerosis, arthritis, lupus, and type I diabetes.
Description:

Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #NM_005018).

Materials Required But Not Provided:
Synonym(s):
Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L, PD1/NFAT, PD-1 NFAT, PD-1/NFAT
Host Cell line:
Jurkat
Mycoplasma Testing:

The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.

Growth Media:

Thaw Medium 2 (BPS Cat. #60184) : RPMI1640 medium (Life Technologies #A10491-01) supplemented with 10% FBS (Life Technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01)

Complete Growth Medium: Thaw Medium 2 (BPS Cat. #60184), 1 mg/ml of Geneticin (Life Technologies #11811031), and 200 μg/ml of Hygromycin B (Hyclone #SV30070.01) Cells should be grown at 37°C with 5% CO2 using complete growth medium (Thaw Medium 2, Geneticin, and Hygromycin B).

Storage / Stability:
Immediately upon receipt, store in liquid nitrogen.
Application(s):
Use this PD-1 Cell Line to screen for activators or inhibitors of PD-1 signaling in a cellular context • Characterize the biological activity of PD-1 and its interactions with ligands
Notes:

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License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Scientific Category:
Immunotherapy
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

60535.pdf