Anti-PD-1 Neutralizing Antibody

Catalog #: 71120
Size: 100 µg
$475
Species: Human/Mouse
Host Species: Freestyle 293 cells
MW: 50 and 25 kDa for the heavy and light chains, respectively (150 kDa).
Tag(s): none
Clonality: Monoclonal
Isotype: IgG1
Description:

Neutralizing recombinant murine/human chimeric antibody recognizing the PD-L1/PD-L2 binding region of human PD-1. This antibody does not cross-react with mouse PD-1, but does cross-react with monkey (M. fascicularis) PD-1. It has not been tested with other species.

UniProt
Q15116
Synonym(s):
Anti-PD1
Specific Activity:
IC50 = 1.2 nM using the PD-1[Biotinylated]:PD-L1 Inhibitor Screening Assay Kit (BPS Cat. #72005). IC50 = 4.7 nM using the PD-1[Biotinylated]:PD-L2 Inhibitor Screening Assay Kit (BPS Cat. #72006).
Assay Conditions:
Antibody was added to PD-1[Biotinylated]:PD-L1 or PD-1[Biotinylated]:PD-L2 binding assay at various concentrations. Assay was performed according to the protocol in BPS Cat. #72005 or #72006, respectively.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Purification:
Protein A affinity chromatography from HEK293 supernatants.
Immunogen:
PD-L1/L2 binding region of human PD-1
Format:
Aqueous buffer solution
Storage / Stability:
Stable for at least 12 months at -80°C. Store diluted antibody with carrier protein (BSA 0.1 – 0.5%) at 4°C, stable for 2 weeks.
Application(s):
Useful as a control inhibitor of PD-1 binding using the PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit, BPS Bioscience, Catalog #72003.
Reference(s):

1. Shinohara, T., et al., Genomics. 1994 Oct;23(3):704-6.

2. James, E.S., et al., Genes Immun. 2005 Aug;6(5):430-7.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Cell Surface Receptor
Product Type:
Antibody
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Product Datasheets

71120_Lot 060420.pdf