PD-1[Biotinylated]:PD-L1 Inhibitor Screening Colorimetric Assay Kit
Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD- 1[Biotinylated]:PD-L1 Inhibitor Colorimetric Screening Assay Kit is designed for screening inhibitors of PD-1 receptor binding to PD-L1 ligand. TThe key to this kit is the high affinity of biotin-labeled PD-1 for streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
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This product has been cited 1 time.
Catalog Number |
Component |
Amount |
Storage |
|
71109 | PD-1, Biotin-labeled (Human)* | 2 µg | -80°C | Avoid freeze/ thaw cycles |
71104 | PD-L1 (Human)* | 10 µg | -80°C | |
79742 | Streptavidin-HRP | 10 µl | +4°C | |
79311 | 3x Immuno Buffer 1 | 50 ml | -20°C | |
79728 | Blocking Buffer 2 | 50 ml | +4°C | |
79651 | Colorimetric HRP substrate | 10 ml | +4°C | |
79964 | Transparent 96-well microplate | 1 | +4°C |
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.