PCSK9[Biotinylated]-LDLR Binding Assay Kit

Catalog #
72002
$805 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. PCSK9 is known to function as a negative regulator of hepatic low-density lipoprotein receptors (LDLRs) by binding to the LDLR ectodomain. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9 by streptavidin-HRP. Only a few steps are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, measured using a chemiluminescence reader.

*NOTE: As of November 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

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This product has been cited 7 times.

Synonyms
pcsk9, cholesterol, LDL, kexin
Product Info
Storage and Usage
Citations7
Assay Kit Format
Chemiluminescent
Supplied As
The PCSK9[Biotinylated]-LDLR Binding Assay Kit comes in a convenient 96-well format, with biotin-labeled PCSK9, purified LDLR ectodomain, streptavidin labeled HRP, and assay buffer for 100 binding reactions.
Materials Required But Not Supplied
  • PBS (Phosphate Buffer Saline)
  • Luminometer or plate reader capable of reading chemiluminescence
  • Rotating or rocker platform
Format
Catalog # Name Amount Storage
71304 PCSK9[Biotinylated]* 10 µg -80°C Avoid multiple freeze/ thaw cycles
71205 LDLR* 10 µg -80°C
79742 Streptavidin-HRP 10 µl +4°C
33298 3x PL-01 Assay Buffer 50 ml -20°C
79728 Blocking buffer 2 50 ml +4°C
79670 ELISA ECL Substrate A (translucent bottle) 6 ml Room Temp
ELISA ECL Substrate B (brown bottle) 6 ml Room Temp
79699 White 96-well microplate 1 Room Temp  

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #
Q8NBP7
Background

PCSK9 regulates circulating cholesterol homeostasis by binding to the ectodomain of liver low-density lipid receptors and promoting their degradation. PCSK9 binds to the low density lipoprotein receptor (LDLR), the very low density lipoprotein receptor (VLDLR), the apolipoprotein E receptor (LRP1/APOER), and the apolipoprotein receptor 2 (LRP8/APOER2).

References

1. Chan, J.C. et al. (2009). Proc. Natl Acad. Sci. USA, 106, 9820-9825.
2. Liang, H., et al. (2012) J. Pharmacol. Exp. Ther. 340 2289-236