Human PCSK9 (proprotein convertase subtilisin/kexin type 9), also known as FH3, HCHOLA3, and PC9, encompassing amino acids 31-692(end). This construct contains a C-terminal His-tag (6xHis) followed by an Avi-tag™. This protein was affinity purified and enzymatically biotinylated using Avi-tag™ technology.
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
MW
Mature: 60 kDa + glycans; Prodomain: 14 kDa
Amino Acids
31–692(end)
Glycosylation
This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Genbank #
NM_174936
UniProt #
Q8NBP7
Tag(s)
C-terminal His, Avi Tags
Label
This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.
For more information on enzymatic biotinylation, please see our Tech Note.
Background
PCSK9 is a crucial player in the regulation of plasma cholesterol homeostasis. It binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a
clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
References
1. Abifadel, M., et al., Nat Genet. 2003 Jun,34(2):154-6. 2. Abifadel, M., et al., Hum Mutat. 2009 Apr;30(4):520-9.
Storage/Stability
At least 6 months at -80°C.
Assay Conditions
Assay was performed according to PCSK9-LDLR TR-FRET Assay Kit (BPS Bioscience #72010) with PCSK9 [Biotinylated] enzyme titrated at various amounts.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
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