PARP3 Chemiluminescent Assay Kit

Catalog #: 80553
Size: 96 reactions
$945
Background:
PARP3 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones.
Description:
The PARP3 Chemiluminescent Activity Assay kit is designed to measure PARP3 activity for screening and profiling applications. The key to the PARP3 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP3 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with the assay buffer that contains the PARP3 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce a chemiluminescence that can then be measured using a chemiluminescence reader.
Synonym(s):
poly (ADP-ribose) polymerase 3, poly(ADP-ribosyl)transferase 3, PARP3
Supplied As:
The PARP3 assay kit comes in a convenient 96-well format, with purified PARP3 enzyme, histone mixture, activated DNA, and PARP assay buffer for 100 enzyme reactions.
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.
Notes:
This kit is also suitable for use with cell extracts, but nonspecific ribosylation activity may be detected.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PARP
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

80553.pdf