PARP11, GST-Tag, His-Tag Recombinant

Catalog #

80511

$490 *

Size: 10 µg

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Description

Recombinant human PARP11, (poly(ADP-ribose) polymerase family member 11), encompassing amino acids 8-338(end). This construct contains an N-terminal GST-tag and a C-terminal His-tag (6xHis). The recombinant protein was affinity purified.

This product has been cited 6 times.

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Synonyms

Protein mono-ADP-ribosyltransferase PARP11, ADP-ribosyltransferase diphtheria toxin-like 11, Poly [ADP-ribose] polymerase 11, C12orf6, PARP-11

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PARP11 Activity

PARP11 inhibition curve using a chemiluminescent assay (#80561). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations6

Species

Human

Construct

PARP11 (GST-8-338(end)-His)

Host Species/Expression System

Sf9

Purity

≥22%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, variable glutathione, 20% glycerol, and 3 mM DTT.

65 kDa

Amino Acids

8-338 (end)

Unit Definition

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.

Genbank #

NM_020367

UniProt #

Q9NR21

Tag(s)

N-terminal GST-tag, C-terminal His-tag

References

1. Rouleau M., et al. J Cell Biochem. 2007 Feb 1;100(2):385-401.
2. Augustin A., et al. J Cell Sci. 2003 Apr 15;116(Pt 8):1551-62.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay done according to PARP11 Chemiluminescent Assay Kit (BPS Bioscience #80561) with various concentrations of PARP11.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PARP

Regulatory Status

Research Use Only

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Datasheets/Protocols

80511 Lot 220421-B

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PARP11, GST-Tag, His-Tag Recombinant

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