PARP1 Chemiluminescent Assay Kit (384-well)

Catalog #: 80569
Size: 384 reactions
$1,050
Species: Human
Description:
The PARP1 Chemiluminescent Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NADdependent addition of poly (ADP-ribose) to histones. The key to the PARP1 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Synonym(s):
poly (ADP-ribose) polymerase 1, poly(ADP-ribosyl)transferase, ADPRT, PARP1, PARP-1
Supplied As:
The PARP1 assay kit comes in a convenient 384-well format, with purified PARP1 enzyme, histone mixture, activated DNA, and PARP1 assay buffer for 400 enzyme reactions.
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt
Format:

Instructions for use:
See assay kit datasheet for detailed protocol.
Storage / Stability:
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Notes:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
1x PBS buffer PBST buffer (1x PBS, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PARP
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

80569.pdf