p38α, GST-tag Recombinant

Catalog #

40070

$380 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human p38α, also known as MARK14, p38 MAP Kinase, and Mitogen- Activated Protein Kinase 14, a.a. 1-360 (end), Genbank Accession No. NM_139012 with an N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW = 68 kDa.

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Synonyms

CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, MAPK14, p38

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Supplied As

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween- 20, 2 mM DTT and 20% Glycerol.

68 kDa

Amino Acids

1-360 (end)

Specific Activity

77 pmol/min/µg

Genbank #

NM_139012

UniProt #

Q16539

Tag(s)

N-terminal GST-tag

Background

p38α (SAPK2A) is a member of the p38 MAPK family which are activated by various environmental stresses and proinflammatory cytokines (1). The activation of p38 requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase (2). The substrates of p38 include transcription regulator ATF2, MEF2C, MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.

References

1. Johnson, G. et al., Science. 298(5600):1911-1912, 2002.
2. Raingeud, J. et al., JBC. 270(13): 7420-7426, 1995.

Storage/Stability

>6 months at -80°C

Assay Conditions

Assay was performed in kinase buffer containing 0.2 mM DTT using P38 peptide substrate (0.1 mg/ml) and 20 μM ATP. Reaction was done at 30 °C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid multiple freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40070_Lot_190128-G2.pdf

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p38α, GST-tag Recombinant

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