P300 Chemiluminescent Assay Kit

Catalog #

79705

$465 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The p300 Chemiluminescent Assay Kit is an enzyme-linked immunosorbent assay (ELISA) designed to screen for inhibitors of p300 or to measure histone acetyltransferase (HAT) activity for screening and profiling applications. Histone acetyltransferase p300 (also known as KAT3B) is involved in various cellular events, and its dysfunction is linked to a number of human diseases including Parkinson’s, Huntington’s, and Alzheimer’s diseases. The P300 Chemiluminescent Assay Kit is based on the p300 enzyme transferring an acetyl group from an acetyl donor (acetyl CoA) to a histone substrate. The acetylated histone is recognized by a highly specific primary antibody, followed by an HRP-labeled secondary antibody. The chemiluminescence produced by HRP can be measured using a chemiluminescence reader.

P300 Chemiluminescent Assay Kit

Need us to run inhibitor screens or profile your compounds against P300? Check out our Acetyltransferase Screening Services.

This product has been cited 1 time.

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Synonyms

E1A Binding Protein P300, Histone Acetyltransferase P300, P300 HAT, Histone Crotonyltransferase P300, Protein Propionyltransferase P300

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Product Data Gallery

Product Info

Storage and Usage

Citations1

Assay Kit Format

Chemiluminescent

Supplied As

The p300 Chemiluminescent Assay Kit comes in a convenient format, with Histone peptide, a 96-well plate precoated with Neutravidin and histone peptide, and all the reagents necessary for 96 chemiluminescent P300 activity measurements. In addition, the kit

Materials Required But Not Supplied

Tween-20
TBST buffer (1 x Tris-Buffered Saline, pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog #	Component	Amount	Storage
50071	P300 human recombinant enzyme	>1 μg	-80°C	Avoid freeze/ thaw cycles!
	Acetyl CoA (0.1 mM)	10 μl	-20°C
79708	2X HAT assay buffer	10 ml	-20°C
	0.5 M DTT	200 μl	-20°C
79706	Primary antibody 327	10 μl	-80°C
52131H	Secondary HRP-Labeled antibody 2	10 μl	-80°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	Room Temp.
79670	ELISA ECL substrate B (brown bottle)	6 ml	Room Temp.
52100	Blocking buffer	50 ml	+4°C
	96-well plate precoated with histone substrate.	1 plate	+4°C

UniProt #

Q09472

References

Ravindra, K. C., et al. (2012). Chem Res Toxicol. 25(2): 337–347.

Storage/Stability

At least 6 months from date of receipt when stored as directed.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt.

Warnings

Avoid multiple freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Acetyltransferase

Regulatory Status

Research Use Only