PI3 kinase p110α(K111N)/p85α Recombinant

Catalog #

40639

$415 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Complex of human p110α, also known as PIK3CA, GenBank Accession No. NM_006218, a.a. 2-1068(full length), with K111N mutation, with N-terminal FLAG-tag, MW=124 kDa, and human p85α, also known as PIK3R1, GenBank Accession No. NM_181523, a.a. 1-724(full length), MW=84 kDa. Coexpressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PIK3CA, p110alpha, Phosphoinositide-3-Kinase, Catalytic, Alpha, PIK3R1, Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha), GRB1, p85alpha, PI3K, pi3k

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 0.2 mM TCEP, 80 μg/ml flag peptide

p110α(K111N): 125 kDa; p85α: 84 kDa

Amino Acids

p110α(K111N): 2-1068(full length); p85α: 1-724(full length)

Specific Activity

1326 pmol/min/μg.

Genbank #

p110α(K111N): NM_006218; p85α: NM_181523

UniProt #

p110α(K111N): P42336; p85α: P27986

Tag(s)

p110α(K111N): N-terminal FLAG-tag

References

1. Rudd, M.L., et al., Clin. Canc. Res. 2011; 17(6): 1331-1340.
2. Brady, S.W., et al., Molec. Canc. Ther. 2014; 13(1): 60-70.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Reactions (25 μl) were done at 30°C for 15 min using BPS PI3K substrate, and the samples were incubated with the ADPGlo reagent (25 μl) for 45 min followed by incubation with the Kinase Detection reagent (50 μl) for 55 min.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Scientific Category

Lipid Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

40639_Lot 150716-1

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PI3 kinase p110α(K111N)/p85α Recombinant

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