OX40L (CD252), His-tag (Human) Recombinant

Catalog #

71185

$350 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human OX40L, also known as tumor necrosis factor ligand superfamily member 4, TNFSF4, and CD252, GenBank Accession No. NM_003326, a.a. 51-183 with a N-terminal His-tag, expressed in a HEK293 cell expression system. MW = 18 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

This product has been cited 1 time.

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Synonyms

tumor necrosis factor ligand superfamily member 4, TNFSF4, CD252

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

OX40[Biotinylated]: OX40L inhibition curve using a chemiluminescent assay (#72045). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations1

Species

Human

Construct

OX40L (His-51-183)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

18 kDa + glycans

Amino Acids

51-183

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Genbank #

NM_003326

UniProt #

P23510.1

Tag(s)

N-terminal His-tag

References

1. Linton, P.J., et al., J. Exp. Med. 2003; 197(7): 875-883.
2. Arestides, R.S.S., et al., Eur. J. Immunol. 2002; 32(10): 2874-2880.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was performed according to OX40[Biotinylated]:OX40L Inhibitor Screening Assay Kit (BPS Bioscience #72045) with OX40 [Biotin] enzyme titrated at various concentrations

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

71185 Lot 220425

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OX40L (CD252), His-tag (Human) Recombinant

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