OX40 / NF-κB Reporter – HEK293 Recombinant Cell Line

Catalog #: 60482
Size: 2 vials
$7,500
Background:
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40, is a member of the TNFR-superfamily of receptors. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Its ligand, OX40L, binds to OX40 receptors on T-cells, preventing them from dying and subsequently increasing cytokine production. OX40 has a critical role in the maintenance of an immune response beyond the first few days and onwards to a memory response due to its ability to enhance survival.
Description:
Recombinant HEK293 cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human OX40 (Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_003327)
Materials Required But Not Provided:

Assay Medium: Thaw Medium 1 (BPS Cat. #60187)
OX40L, His tag (BPS Cat. #71185)
Anti-OX40 antagonist antibody (BPS Cat. #72063)
96-well tissue culture-treated white clear-bottom assay plate
One-Step luciferase assay system (BPS Cat. #60690) or other luciferase reagents for measuring firefly luciferase activity
Luminometer

Synonym(s):
OX40, OX40 cell Line, CD134 Cell Line, ox40 nfkb
Host Cell line:
HEK293
Mycoplasma Testing:
The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Format:
Each vial contains 2 x 10^6 cells in 1 ml of 10% DMSO
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Application(s):
Screen for activators or inhibitors of OX40 signaling in a cellular context and characterize the biological activity of OX40 and its interactions with ligands
Notes:

Contact us for pricing.

License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Scientific Category:
Immunotherapy
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

60482.pdf