OX40 - HEK293 Recombinant Cell Line

Catalog #: 60682
Size: 2 vials
OX40 (CD134) is a co-stimulatory receptor expressed on the surface of CD4+ and CD8+ T cells 24 to 48 hours after activation. Binding of OX40 to its ligand, OX40L (CD252), present on dendritic cells, potentiates T cell survival and increases cytokine production. OX40 has been shown to activate NF-κB-mediated memory cell generation through its interaction with adaptor proteins TRAF2 and TRAF5. OX40 has a critical role in the maintenance of an immune response beyond the first few days and onwards to a memory response.
Recombinant HEK293 stably expressing human OX40 (TNF receptor superfamily member 4, TNFRSF4, ACT35; CD134; IMD16; TXGP1L, GenBank Accession #NM_003327).
TNFRSF4, ACT35, IMD16, TXGP1L, Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, CD134
Supplied As:
Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO
Host Cell line:
Mycoplasma Testing:
The cell line has been screened using the metabolite-based Mycoplasma Detection Kit (Biotool #B3903) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 1 (BPS, #60187): MEM medium (Hyclone, #SH30024.01) supplemented with 10% FBS (Invitrogen, #26140-079), 1% non-essential amino acids (Hyclone, #SH30238.01), 1 mM Na pyruvate (Hyclone, #SH30239.01), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01).

Growth Medium 1F (BPS, #79540): Thaw Medium 1 (BPS, #60187) plus 100 µg/ml Hygromycin B (Thermo Fisher, #10687010).

Instructions for use:
See data sheet for detailed culturing and assay protocol
Storage / Stability:
Immediately upon receipt, store in liquid nitrogen.
OX40 binding molecule (such as anti-OX40 antibody) screening and profiling in a cellular context

1. Rogers, P.R. et al., Immunity. 2001; 15(3): 445-455.

2. Ito, T. et al., J. Exp. Med. 2005; 202(9): 1213-1223.


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License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Avoid freeze/ thaw cycles.
Scientific Category:
Product Type:
Cell Line
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