OX40[Biotinylated]:OX40L Inhibitor Screening Assay Kit

Catalog #: 72045
Size: 96 reactions
$1,040
Background:
OX40 (CD134) is a co-stimulatory receptor expressed on the surface of T cells. Binding of OX40 to its ligand, OX40L (CD252), present on dendritic cells, potentiates T cell activation and T cell effector function. Studies have shown that OX40 agonists can increase anti-tumor immunity and improve tumor-free survival in pre-clinical studies. Alternatively, OX40 antagonists offer potential as therapeutics for inflammatory diseases.
Description:
The OX40[Biotinylated]:OX40L Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of OX40:OX40L interaction. The key to this kit is the high sensitivity of detection of biotinlabeled OX40 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, OX40L is coated on a 96-well plate. Next, OX40-biotin is incubated with OX40L on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
Synonym(s):
Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, CD134, tumor necrosis factor ligand superfamily member 4, TNFSF4, CD252
Supplied As:
This kit comes in a convenient 96- well format, with biotin-labeled OX40, purified OX40L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
This kit is useful for screening for inhibitors of OX40 binding to OX40L.
Reference(s):

1. Peng, K., et al. 2014; AAPS J. 16(4): 625–633.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

72045.pdf