OTUD6B, His-FLAG-tags Recombinant

Catalog #

80407

$425 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human deubiquitinating enzyme ovarian tumor domain-containing 6B, or OTUD6B (GenBank Accession No. NM_016023), a.a. 2-323 (end) with N-terminal His-FLAG-tag, MW=39 kDa, expressed in E. coli cells.

Need us to run inhibitor screens or profile your compounds against OTUD6B? Check out our Protease Screening Services or Ubiquitination Screening Services.

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Synonyms

DUB, OTUD6B, ovarian tumor domain

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, 20% glycerol and 3 mM DTT.

39 kDa

Amino Acids

2–323 (end)

Specific Activity

24 pmole/min/mg

Genbank #

NM_010623

UniProt #

Q8N6M0

Tag(s)

N-terminal His-FLAG-tags

References

1. Xu, Z. et al., PLoS One. 2011 Jan 18; (6)1: e14514.
2. Sowa, ME. et al., Cell. 2009 Jul 23; 138(2): 389-403.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Reaction was performed in 50 mM Tris pH 7.4, 1 mM DTT. 0.5 mM EDTA, 500 nM Ub-AMC and OTUD6B. Reaction was incubated at 37°C for 15 min and fluorescent signal was measured at excitation of 340 nm and emission at 460 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitins: Deubiquitinase (DUB)

Regulatory Status

Research Use Only

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Datasheets/Protocols

80407_Lot111014FH.pdf

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OTUD6B, His-FLAG-tags Recombinant

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