Sirtuin 2, His-tag

Catalog #: 50013
Size: 100 µg
$405.00
Species: Human
Host Species: E. coli
MW: 35.5 kDa
Genbank #: NM_012237
Tag(s): C-terminal His-tag
a.a: 13–319
Description:
Human Sirtuin 2, GenBank Accession No. NM_012237, a.a. 50-356 with C-terminal His-tag, MW= 35.5 kDa, expressed in an E. coli expression system.
UniProt
Q8IXJ6
Synonym(s):
Sirtuin2, SIRT2
Specific Activity:
≥1.0 pmol/min/µg.
Assay Conditions:
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 1 (Catalog #50032), 500 µM NAD+ and Sirtuin 2 (125 - 8000 ng). Incubation condition: 30 min at 37°C followed by treatment with 50 µl of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.
Formulation:
40 mM Tris pH 8.0, 110 mM NaCl, 2.1 mM KCl, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Grubisha, O., 2006, J.Biol.Chem. 281, 20, 14057-65.
2. Vaquero, A., 2006, Genes Dev. 20, 10, 1256- 61.

Product Specific Reference(s): 
1. Nguyen, P., 2014, Mol Cancer Res. 12, 9, 1244-53. 

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50013_Lot150501-G.pdf