JHDM1D (KDM7A), FLAG-tag

Catalog #: 50419
Size: 20 µg
$425.00
Species: Human
Host Species: Sf9 Insect Cells
MW: 56 kDa
Genbank #: NM_030647
Tag(s): N-terminal FLAG-tag
a.a: 2-488
Description:
Human JHDM1D, also known as Lysine-specific demethylase 7A (KDM7A) and KIAA1718, GenBank Accession No. NM_030647 a.a. 2-488 with an N-terminal FLAG-tag, MW=56 kDa, expressed in a baculovirus-infected Sf9 cell expression system.
UniProt
A0JNV9
Synonym(s):
Lysine-specific demethylase 7A, KDM7A, KIAA1718
Specific Activity:
0.2 pmole/min/μg
Assay Conditions:
Assay Conditions: 10 μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 μM alpha-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 μM biotinylated peptide substrate, and JHDM1D (8 – 40ng) added to the wells. Add antibody against monomethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 μg/ml FLAG peptide
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Yang, C., et al. Cell Res. 2010. 20: 886-898.
2. Osawa, T. et al. Proc. Nat. Acad. Sci. (USA). 2011. 108(51): 20725-20729.

Warning(s):
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Scientific Category:
Demethylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

50419_Lot_160208.pdf