JARID1A (KDM5A), FLAG-tag

Catalog #: 50110
Size: 20 µg
$450.00
Species: Human
Host Species: Sf9 insect cells
MW: 125 kDa
Genbank #: NM_001042603
Tag(s): C-terminal FLAG-tag
a.a: 1–1090
Description:
Human JARID1A, also known as KDM5A and RBBP2, GenBank Accession #NM_001042603), a.a. 1-1090 with C-terminal FLAG-tag, MW= 125 kDa, expressed in Sf9 cells via a baculovirus expression system.
UniProt
P29375
Synonym(s):
KDM5A, RBBP2, JARID1A
Specific Activity:
0.024 pmole/min/μg
Assay Conditions:
10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JARID1A (80 – 400 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 µg/ml FLAG peptide
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Roesch, A., et al., (2005). Mod Pathol. 18(9):1249-57.
2. Zhou, X., et al., (2010). Cancer Res. 70(10):4214-21
3. Van Zutven, LJ., et al., (2006). Genes Chromosomes Cancer 45(5):437-46.

Application Reference(s):
A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Demethylase
Product Type:
Recombinant Protein
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Product Datasheets

50110_Lot_140501-1.pdf