HDAC8, His-tag

Catalog #: 50008
Size: 50 µg
$410.00
Species: Human
Host Species: Sf9 insect cells
MW: 42.4 kDa
Genbank #: NM_018486
Tag(s): C-terminal His-tag
a.a: full length
Description:
Human HDAC8 , GenBank Accession No. NM_018486, full length with C-terminal His-tag, MW= 46.4 kDa, expressed in a baculovirus expression system.
UniProt
Q9BY41
Synonym(s):
histone deacetylase 8, HDAC-8
Specific Activity:
≥300 pmol/min/µg.
Unit Definition:
One U =1 pmol of acetyl group removed/min/µg of enzyme
Assay Conditions:
25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC8 (3.1 - 200 ng). Incubation condition: 30 min at 37°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, and 10% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
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Product Datasheets

50008_Lot131120-1.pdf