HDAC6, GST-tag

Catalog #: 50006
Size: 50 µg
$410.00
Species: Human
Host Species: Sf9 insect cells
MW: 159 kDa
Genbank #: BC069243
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Human HDAC6 protein, GenBank Accession No. BC069243, full length with N-terminal GST-tag, MW= 159 kDa, expressed in baculovirus expression system.
UniProt
Q9UBN7
Synonym(s):
histone deacetylase 6, HDAC-6, HDAC6 Protein
Specific Activity:
≥450 pmol/min/µg.
Assay Conditions:
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 μM BPS HDAC substrate 3 (Catalog #50037), and HDAC6 (3.1 – 200 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature.
Formulation:
50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 50 mM KCl, 2 mM EDTA, 10% glycerol, and 2 mM DTT
Purification:
Protein was purified by affinity chromatography and gel filtration.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Li S. et al., Neurology. 41(2), 112-6 (2010).
2. Strausberg, R.L. et al., Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002).

Application References:
1. Novel analogs targeting histone deacetylase suppress aggressive thyroid cancer cell growth and induce re-differentiation (2015)
2. Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014)
3. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014)
4. Kinetic method for the large-scale analysis of the binding mechanism of histone deacetylase inhibitors (2014)
5. Thermodynamics of ligand binding to histone deacetylase like amidohydrolase from Bordetella/Alcaligenes (2014)
6. A selective HDAC 1/2 inhibitor modulates chromatin and gene expression in brain and alters mouse behavior in two mood-related tests (2013)
7. Crebinostat: a novel cognitive enhancer that inhibits histone deacetylase activity and modulates chromatin-mediated neuroplasticity (2013)
8. Highly ligand efficient and selective N-2-(Thioethyl)picolinamide histone deacetylase inhibitors inspired by the natural product psammaplin A (2013)
9. Potent histone deacetylase inhibitors derived from 4-(aminomethyl)-N-hydroxybenzamide with high selectivity for the HDAC6 isoform (2013)
10. Santacruzamate A, a potent and selective histone deacetylase inhibitor from the Panamanian marine cyanobacterium cf. Symploca sp (2013)
11. Thioester derivatives of the natural product psammaplin A as potent histone deacetylase inhibitors (2013)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
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Product Datasheets

50006_Lot150609.pdf