HDAC3/NcoR2 Complex, His-tag

Catalog #: 50003
Size: 50 µg
Species: Human
Host Species: Sf9 insect cells
MW: 87.3 kDa (complex)
Genbank #: NM_003883
Tag(s): C-terminal His-tag
a.a: full length HDAC3, 395– 489 NCOR2
Complex of human HDAC3 , GenBank Accession No. NM_003883, full length with C-terminal His-tag, MW= 49.7 kDa, and human NCOR2 , a.a. 395- 489, GenBank Accession No. NM_006312, N-terminal GST-tag, MW= 37.6 kDa, co-expressed in baculovirus expression system.
histone deacetylase 3, HDAC-3
Specific Activity:
≥3000 pmol/min/µg.
Assay Conditions:
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3 (Catalog #50037), and HDAC3/NcoR2. Incubation condition: 30 min at 37°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 200 mM imidazole
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Gregoire, S. et al. Mol. Cell. Biol. 27 (4), 1280-1295 (2007)
2. Escaffit, F. et al., Mol. Cell. Biol. 27 (2), 554-567 (2007)

Application References:
1. Identification of histone deacetylase inhibitors with benzoylhydrazide scaffold that selectively inhibit class I histone deacetylases (2015)
2. Mechanism of Action of 2-Aminobenzamide HDAC Inhibitors in Reversing Gene Silencing in Friedreich's Ataxia (2015)
3. Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014)
4. Discovery of a small molecule agonist of phosphatidylinositol 3-kinase p110α that reactivates latent HIV-1 (2014)
5. A chimeric SERM-histone deacetylase inhibitor approach to breast cancer therapy (2014)
6. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014)
7. Selective HDAC Inhibition for the Disruption of Latent HIV-1 Infection (2014)
8. Class I HDAC imaging using [ (3)H]CI-994 autoradiography (2013)
9. Crebinostat: a novel cognitive enhancer that inhibits histone deacetylase activity and modulates chromatin-mediated neuroplasticity (2013)
10. FDG-PET imaging reveals local brain glucose utilization is altered by class I histone deacetylase inhibitors (2013)
11. Highly ligand efficient and selective N-2-(Thioethyl)picolinamide histone deacetylase inhibitors inspired by the natural product psammaplin A (2013)
12. Imaging epigenetic regulation by histone deacetylases in the brain using PET/MRI with ¹⁸F-FAHA (2013)
13. Structural basis for the inhibition of histone deacetylase 8 (HDAC8), a key epigenetic player in the blood fluke Schistosoma mansoni (2013)

Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Recombinant Protein
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