RARγ Luciferase Reporter HEK293 Cell Line
RARγ Luciferase Reporter HEK293 Cell Line is a HEK293 cell line engineered to express firefly luciferase, under the control of retinoic acid response elements (RARE), and with full length human RARγ (retinoic acid receptor gamma) (accession # P13631-1). RARγ Luciferase Reporter HEK293 Cell Line is designed for monitoring the activity of RARγ. This cell line was functionally validated by stimulation with all-trans retinoic acid and Western Blot analysis.
Created with BioRender.com
Figure 1: Illustration of the mechanism of action of RARγ Luciferase Reporter HEK293 Cell Line. Upon activation with retinoic acid, RAR dimerizes with RXR, and activates RARE and luciferase expression. The level of activation corresponds directly to the luciferase signal.
Interested in screening and profiling inhibitors or activators of retinoic acid receptor gamma without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.
This product has been cited 1 time.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Media Required for Cell Culture
Name | Ordering Information |
Thaw Medium 6 | BPS Bioscience #60183 |
Growth Medium 6A | BPS Bioscience #79542 |
Materials Used in the Cellular Assay
Name | Ordering Information |
ATRA | Sigma #R2625 |
Assay Medium 6A | BPS Bioscience #82211 |
96-well tissue culture treated white clear-bottom assay plate | Corning #3610 |
ONE-Step™ Luciferase Assay System | BPS Bioscience #60690 |
Luminometer |
The cell line has been screened to confirm the absence of Mycoplasma species.
Retinoic acid receptor gamma (RARγ), also known as NR1B3 (nuclear receptor subfamily 1, group B, member 3) belongs to the family of nuclear receptors and has three subtypes, RARa, RARb, and RARg. RAR heterodimerizes with retinoic X receptor (RXR) and acts as a transcription factor that regulates the growth and differentiation of both normal and malignant cells. When RAR binds to its ligands, all-trans retinoic acid or 9-cis retinoic acid, RAR/ RXR heterodimer binds to retinoic acid response elements in the promoter region of target genes and recruits coactivator proteins, leading to transcription and expression of the downstream target genes. RARγ can be found in T cells and it is involved in thymocyte development and CD8+ T cell responses. It is present at high levels in hematopoietic stem cells during hematopoiesis. It can act as an oncogene, leading to colorectal, renal and hepatocellular carcinoma and acute myeloid leukemia (AML). The development of RAR antagonists, either selective or pan-specific, may prove a useful treatment option in cancer therapy.
Petkovich M., et al., 1987 Nature 330(6147): 444-450.
Allenby G., et al., 1993 Proc. Natl. Acad. Sci. USA 90(1): 30-34.
Brown G. and Petrie K., 2021 Int J Mol Sci 22(7):3632.