RARα Luciferase Reporter HEK293 Cell Line

Catalog #

60503

$2,240 *

Size: 2 vials

Qty

Bulk/Custom

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Description

RARα Luciferase Reporter HEK293 Cell Line are engineered HEK293 cells expressing a conditional firefly luciferase reporter under the control of retinoid acid response elements (RARE), and constitutively expressing full length human Retinoic Acid Receptor alpha (RARα, NM_0.00964). This cell line is functionally validated to respond to all-trans retinoic acid (ATRA) stimulation.

Figure 1: Activation of retinoid acid-dependent luciferase reporter in the RARα/RARα Luciferase Reporter HEK293 Cell Line.

Interested in screening and profiling inhibitors or activators of Retinoic Acid Receptor alpha without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 5 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

RAR alpha, NCOR2, SMRT, RARA, Retinoic acid receptor alpha

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Product Data Gallery

Dose response of RARα Reporter – HEK293 Recombinant cells to all-trans retinoic acid (ATRA).

Product Info

Storage and Usage

Citations5

Host Cell Line

HEK293, Human Embryonic Kidney, epithelial-like cells, adherent.

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 6	BPS Bioscience #60183
Growth Medium 6A	BPS Bioscience #79542

Materials Required for Cellular Assay

Name	Ordering Information
ATRA	Sigma #R2625
Assay Medium 6A	BPS Bioscience #82211
96-well tissue culture treated white clear-bottom assay plate	Corning #3610
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

Genbank #

NM_000964

UniProt #

P10276

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

RAR (Retinoic Acid Receptor) belongs to a family of nuclear receptors and has three subtypes: RARa, RARb, and RARg. RAR heterodimerizes with RXR (Retinoid X Receptor) and acts as a transcription factor that regulates the growth and differentiation of both normal and malignant cells. When RAR binds to its ligands, all-trans retinoic acid (ATRA) or 9-cis retinoic acid, the RAR/ RXR heterodimer binds to the retinoic acid response elements in the promoter region of target genes. This recruits coactivator proteins, leading to transcription and expression of the downstream target genes. Retinoic acid (a derivative of Vitamin A) signaling plays critical roles in embryonic and immune system development. Mutations in RARα can lead to Acute Promyelocytic Leukemia (APL), characterized by the accumulation of progenitor cells from the blood lineage (promyelocytes), and other cancer types. Use of RA may prove beneficial in cancer therapy.

References

1. Petkovich, M, et al. Nature (1987) 330(6147): 444-450.
2. Allenby, G, et al. Proc. Natl. Acad. Sci. USA (1993) 90(1): 30-34.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 6 (BPS Bioscience #60183): DMEM supplemented with 10% FBS and 1% Penicillin/Streptomycin.

Growth Medium 6A (BPS Bioscience #79542): DMEM supplemented with 10% FBS, and 1% Penicillin/Streptomycin plus 400 µg/ml of Geneticin (G418), 1 µg/ml Puromycin and 100 µg/ml Hygromycin.

Media Required for Functional Cellular Assay 
Assay Medium 6A (BPS Bioscience #82211): Phenol red-free DMEM, 10% Charcoal Stripped FBS, and 1% Penicillin/Streptomycin.

Applications

Monitor RARα-regulated pathway activity.
Screen RARα agonists and antagonists.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Nuclear Receptor

Regulatory Status

Research Use Only