NSD3 Chemiluminescent Assay Kit

Catalog #

79358

$1,950 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The NSD3 Chemiluminescent Assay Kit is designed to measure NSD3 activity for screening and profiling applications. The key to the NSD3 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

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Synonyms

WHSC1L1, Protein Whistle, NSD3, Nuclear SET Domain-Containing Protein 3, Wolf-Hirschhorn Syndrome Candidate 1-Like 1, NSD

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Product Data Gallery

NSD3 Inhibition by Chaetocin

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

The NSD3 Chemiluminescent Assay Kit comes in a convenient format, with 384-well plate precoated with a specific substrate, the antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransfer

Materials Required But Not Supplied

• TBST buffer (1x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)

• Luminometer or microplate reader capable of reading chemiluminescence

• Adjustable micropipettor and sterile tips

• Rotating or rocker platform

Format

Catalog #	Component	Amount	Storage
51036	NSD3 human enzyme	200 µg	-80°C	Avoid freeze/ thaw cycles
	100 µM S-adenosylmethionine	2 x 250 µl	-80°C
52140P2	Primary antibody 16-2	2 x 12.5 µl	-80°C
52131H	Secondary HRP-labeled antibody 2	2 x 10 µl	-80°C
52193Z	4x HMT assay buffer 7	2 x 3 ml	-20°C
52100	Blocking buffer 4	2 x 50 ml	+4°C
	HRP chemiluminescent substrate A (translucent bottle)	2 x 6 ml	+4°C
	HRP chemiluminescent substrate B (brown bottle)	2 x 6 ml	+4°C
	Microplate pre-coated with substrate	1	+4°C

UniProt #

Q9BZ95

References

Dillon, S.C., et al. Genome Biology 2005; 6:227.

Bennet, R.L., et al. Cold Spring Harb Perspect Med. 2017; 7(6): a026708.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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NSD3 Chemiluminescent Assay Kit

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