NSD3 Chemiluminescent Assay Kit
The NSD3 Chemiluminescent Assay Kit is designed to measure NSD3 activity for screening and profiling applications. The NSD3 Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase assay buffer, and purified NSD3 enzyme for 96 enzyme reactions. The key to the NSD3 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
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TBST buffer (1x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform
Catalog # | Component | Amount | Storage | |
51036 | NSD3 Human Enzyme | 25 µg | -80°C | Avoid freeze/ thaw cycles! |
100 μM S-adenosylmethionine | 250 µl | -80°C | ||
52140P2 | Primary antibody 16-2 | 12.5 µl | -80°C | |
52131H | Secondary HRP-labeled antibody 2 | 10 µl | -80°C | |
52193Z | 4x HMT assay buffer 7 | 3 ml | -20°C | |
52100 | Blocking buffer 4 | 50 ml | +4°C | |
79670 | ELISA ECL substrate A (translucent bottle) |
6 ml | Room Temp | |
ELISA ECL substrate B (brown bottle) |
6 ml | Room Temp | ||
Microplate pre-coated with substrate | 1 | +4°C |
*The concentration of NSD3 is lot-specific and will be indicated on the tube containing the enzyme