NSD2 Chemiluminescent Assay Kit

Catalog #
79359
$1,100 *
Size: 384 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The NSD2 Chemiluminescent Assay Kit is designed to measure NSD2 activity for screening and profiling applications. The key to the NSD2 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Synonyms
WHSC1, Wolf-Hirschhorn Syndrome Candidate 1, MMSET, Multiple Myeloma SET Domain Containing Protein, NSD2, Nuclear SET Domain-Containing Protein 2, REIIBP, WHS, TRX5, NSD
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Supplied As
The NSD2 Chemiluminescent Assay Kit comes in a convenient format, with 384-well plate precoated with a specific substrate, the antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransfer
Materials Required But Not Supplied

• TBST buffer (1x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20)

• Luminometer or microplate reader capable of reading chemiluminescence

• Adjustable micropipettor and sterile tips

• Rotating or rocker platform

Format
Catalog # Component Amount Storage
51026 NSD2 human enzyme* 50 µg -80°C Avoid freeze/ thaw cycles!
52120 100 µM S-adenosylmethionine 2 x 250 µl -80°C
52140P2 Primary antibody 16-2  2 x 12.5 µl -80°C
52131H Secondary HRP-labeled antibody 2 2 x 10 µl -80°C
52193Z 4x HMT assay buffer 7 2 x 3 ml -20°C
52100 Blocking buffer 4 2 x 50 ml +4°C
  HRP chemiluminescent substrate A (translucent bottle)  2 x 6 ml +4°C
  HRP chemiluminescent substrate B (brown bottle) 2 x 6 ml +4°C
  Microplate pre-coated with substrate 1 +4°C

*The concentration of NSD2 is lot-specific and will be indicated on the tube containing the enzyme  

UniProt #
O96028
References

Dillon, S.C., et al. Genome Biology 2005; 6:227.

Bennet, R.L., et al. Cold Spring Harb Perspect Med. 2017; 7(6): a026708.