Mouse Notch1 Pathway Reporter Kit (Notch Signaling Pathway)

Catalog #: 60509
Size: 500 reactions
The Notch signaling pathway controls cell fate decisions in vertebrate and invertebrate tissues. Notch signaling is triggered through the binding of a transmembrane ligand, such as Delta like ligand 4 (DLL4) or jagged 1 (JAG1) to a Notch transmembrane receptor (Notch1, Notch2, Notch3, Notch4) on a neighboring cell. This results in proteolytic cleavage of the Notch receptor, releasing the constitutively active intracellular domain of Notch (NICD). NICD translocates to the nucleus and associates with transcription factors CSL (CBF1, RBPJ, Suppressor of Hairless, Lag-1) and the coactivator, Mastermind, to turn on transcription of Notch responsive genes.

Notch Pathway Reporter kit is designed for monitoring the activity of the Notch signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for mouse NOTCH1 that has a deletion of the entire extracellular domain, leaving the transmembrane and intracellular regions intact (Notch1DE). Inside the cells, the NOTCH1 DE is cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency.

The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity.

This kit contains the expression vector for Mouse Notch1DE. We also offer the Human Notch1DE expression vector (BPS 79503), as well as a Mouse Notch1 NICD expression vector which is constitutively active without processing by γ-secretase (BPS Bioscience #79504).

Notch reporter, PKA reporter, cAMP reporter, luciferase reporter , CSL reporter, CBF1 reporter, RBP-J reporter, Cell based assay kit, cell-based assay, reporter assay kit
The Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each). The Notch1 Expression Vector and Negative Control Expression Vector are provided at 100 ng DNA/µl (250 µl each).



Instructions for use:
See data sheet for detailed transfection and assay protocols.
Storage / Stability:
Stable at least 12 months from date of receipt, when stored as directed (-20°C)

• Screen for activators or inhibitors of the Notch signaling pathway.
• Study effects of RNAi or gene overexpression on the activity of the Notch pathway.

1. Lu FM et al. (1996). Proc. Natl. Acad. Sci. USA 93(11): 5663-5667.
2. Kanungo et al (2008). J.Neurochem. 106 2236.

Application Reference:
MicroRNA-126-5p promotes endothelial proliferation and limits atherosclerosis by suppressing Dlk1 (2014)

The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.
Avoid freeze/thaw cycles
Scientific Category:
Cell Signaling Pathway
Product Type:
Reporter Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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