NMNAT1, His-Tag Recombinant

Catalog #

71090

$390 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Recombinant human NMNAT1 (nicotinamide nucleotide adenylyltransferase 1), full length encompassing amino acids 2-279 (end). The construct contains an N-terminal His-tag (6xHis). The protein was affinity purified.

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Synonyms

nicotinamide nucleotide adenylyltransferase 1, NMNAT1, NMN adenylyltransferase 1, NMNAT, PNAT1, SHILCA, LCA9

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

NMNAT1 Activity

NMNAT1 inhibition curve using a luminescent assay (#79642). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

NMNAT1 (His-2-279(end))

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, variable Imidazole, 0.04% Tween-20, and 20% glycerol

33 kDa

Amino Acids

2-279(end)

Specific Activity

≥2,300 pmol/min/µg

Genbank #

NM_022787

UniProt #

Q9HAN9

Tag(s)

N-terminal His-tag

References

1. Kaplan, J., et al., Med Sci (Paris). 2013; 29(1):26-27
2. Chiang, P.W., et al., Nat Genet. 2012; 44(9):972-974

Storage/Stability

At least 6 months at –80°C

Assay Conditions

Assay was done according to NMNAT1 Assay Kit (BPS Bioscience #79642) with various amounts of NMNAT1.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes-Transferase

Regulatory Status

Research Use Only

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Datasheets/Protocols

71090 Lot 230322-2

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