NF-κB Reporter Kit (NF-κB Signaling Pathway)

Catalog #: 60614
Size: 500 reactions
NF-κB (Nuclear Factor-KappaB, NFkB) is a heterodimeric protein composed of different combinations of members of the Rel family of transcription factors. The NF-kB /Rel family of transcription factors (p50, p65, c-Rel, etc.) are involved in stress, immune, and inflammatory responses. In unstimulated cells, the NF-κB dimers are sequestered in the cytoplasm by inhibitory IkB proteins. Proinflammatory cytokines, LPS, growth factors, and antigen receptors activate I B kinase (IKK), which phosphorylates the I B proteins. Phosphorylation of IkB leads to its degradation, freeing NF-κB complexes to translocate to the nucleus, bind to NF-κB DNA response elements, and induce the transcription of the target genes.
The NF-κB Reporter kit is designed for monitoring the activity of the NF-κB signaling pathway in the cultured cells. The kit contains transfection-ready NF-κB luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of multimerized NF-κB responsive element located upstream of a minimal promoter. The NF-κB reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.
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Supplied As:
Reporter: NF-κB luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector

Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)

assay kit
Instructions for use:
See data sheet insert for detailed transfection and assay protocols.
Storage / Stability:

Stable at least 12 months from date of receipt, when stored as directed (-20°C)

• Monitor NF-κB signaling pathway activity.
• Screen for activators or inhibitors of NF-κB signaling or NF-κB pathway components.
• Study effects of RNAi or gene overexpression on the activity of the NF-κB pathway.
The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.
Avoid freeze/thaw cycles
Product Type:
Reporter Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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