PDE10A2, GST-tag

Catalog #: 60100
Size: 10 µg
$425
Species: Human
Host Species: Sf9 insect cells
MW: 116 kDa
Genbank #: NM_006661
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Human PDE10A2 (GenBank Accession No. NM_006661), full length with N-terminal GST-tag, MW= 116 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q9Y233
Synonym(s):
PDE10A2, phosphodiesterase 10A2
Specific Activity:
≥ 1600 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3' , 5' -cAMP to 5' -AMP per minute at 37°C.
Assay Conditions:
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 μM cAMP, and serial dilutions of PDE3A at 37ºC for 20 min. Quantified by 5 nucleotidase cleaving the 5-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween, 3mM DTT, and 20% Glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Gross-Langenhoff,M., et al., J. Biol. Chem. 281 (5), 2841-2846 (2006).
2. Frame,M., et al., Cell. Signal. 13 (10), 735- 741 (2001).
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
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Product Datasheets

60100_Lot140507-G1.pdf