Rat PDE10A, GST-tag

Catalog #: 60102
Size: 5 µg
$425
Species: Rat
Host Species: Sf9 insect cells
MW: 66 kDa
Genbank #: NM_022236
Tag(s): N-terminal GST-tag
a.a: 449–end
Description:
Rat PDE10A GenBank Accession No. NM_022236), a.a. 449-end with N-terminal GST-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q9QYJ6
Synonym(s):
PDE10, phosphodiesterase 10A
Specific Activity:
≥ 8339 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3' , 5' -cAMP to 5' -AMP per minute at 37°C.
Assay Conditions:
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 1 mM MnCl2, 200 µM cAMP, and 2.5 kU 5?- nucleotidase, 37°C, 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Siuciak, J.A. et al., Neuropharmacology 51 (2), 374-385 (2006).
2. Hu,H. et al., Eur. J. Neurosci. 20 (12), 3351-3363 (2004).
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
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Product Datasheets

60102_Lot80916.pdf