Mouse PDE10A, GST-tag

Catalog #: 60101
Size: 10 µg
Species: Mouse
Host Species: Sf9 insect cells
MW: 117 kDa
Genbank #: NM_011866
Tag(s): N-terminal GST-tag
a.a: full length
Mouse PDE10A GenBank Accession No. NM_011866), full length with N-terminal GST-tag, MW=117 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
PDE10, phosphodiesterase 10A
Specific Activity:
≥ 1,029 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3' , 5' -cAMP to 5' -AMP per minute at 37°C.
Assay Conditions:
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5 -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
1. Siuciak, J.A. et al., Neuropharmacology 51 (2), 374-385 (2006).
2. Hu,H. et al., Eur. J. Neurosci. 20 (12), 3351-3363 (2004).
Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Recombinant Protein
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